EasyLife TCSPC

DeltaPro™ Software

 
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Don't let the low price of the DeltaPro™ fool you. This low cost system comes with the same world-leading TCSPC decay acquisition and analysis software included with the most advanced HORIBA TCSPC research systems.

World-Leading Decay Analysis Software

HORIBA Scientific decay analysis software recovers kinetic information (such as fluorescence lifetime and rotational correlation times) from luminescence decay data by fitting the raw decay data to one of a selection of kinetic models. Features such as shift iteration and reconvolution of the instrument response function IRF, or prompt, allow the accurate recovery of multiple decay components, even when the decay data is grossly distorted by the IRF.

DAS6, the latest version of our decay analysis software, uses proprietary hybrid grid-search algorithms that rigorously avoid false minima in chi-squared and erroneous decay constants. The license comprises the main application workspace, and allows reconvolution analysis with up to five exponentials. It also includes anisotropy, batch analysis, lifetime distribution, micellar quenching and energy transfer.

Functionality

HORIBA Scientific DAS6 is designed to streamline the analysis of time-domain luminescence data, while still allowing the opportunity for fine-tuning of parameters where necessary. The DAS6 lifetime fitting module contains reconvolution analysis with up to 5 exponentials, as well the following more advanced fitting modules.

Batch mode

Batch mode analyzes datasets containing up to 10,000 decays and is ideal for applications where large amounts of lifetime data are generated e.g. lifetime imaging and reaction monitoring. This module features full reconvolution with up to five exponentials and shift iteration. Parameters can be held common between datasets, for ‘quasi-global analysis’. All data and results are stored in a single data file and results are automatically tabulated for use in external spreadsheet applications.

Global analysis

Full Global analysis of up to 5 exponential components and 100 decay curves.

Lifetime distribution

We offer three distributions analysis models to choose from.

The Top-hat model fits a distribution of decay times and distribution plus additional exponential.

The Maximum Entropy Method (MEM) is designed to recover lifetime distributions without any a priori assumptions about their shapes. This method uses a series of exponentials (up to 200 terms) as a probe function with fixed, logarithmically-spaced lifetimes and variable pre-exponentials.

Our Non-extensive decay (NED) analysis is based on the gamma function distribution, which is the most probable form of distribution for positive lifetimes. Unlike “model free” analyses, it starts with a defined number of distributions. This constraint means that it is less likely to produce artifacts and is a simpler method to fit both complex as well as purely discrete exponential decays.

HSA excited at 295nm, with the decay of the tryptophan modelled using NED distribution analysis

HSA excited at 295nm, with the decay of the tryptophan modeled using NED distribution analysis

Fit to exponential series

Exponential series analysis of up to 30 terms.

Time-resolved anisotropy

Impulse reconvolution of up to 2 correlation times and 5 fluorescence decay components. Each model parameter, including shift, can be fixed at a pre-determined value or optimized as part of the parameter fitting. The use of reconvolution allows accurate determination of decay times and rotational correlation times even when the observed decay is grossly distorted by the duration of the excitation pulse.

FRET energy transfer

2-D and 3-D FRET-type energy transfer with optional additional exponential component.

Yokota-Tanimoto energy transfer

Yokoto-Tanimoto energy transfer with optional additional exponential component.

Micellar quenching

Micellar quenching kinetics.

Unique HORIBA Software Benefits

DeltaPro TCSPC check!
Proven fit algorithms with reconvolution of IRF (prompt)
DeltaPro TCSPC check!
Shift iteration - compensates for wavelength and quantization shifts
DeltaPro TCSPC check!
Unique grid-search algorithm for superior chi-squared minimization (fewer false minima than general purpose Marquardt)
DeltaPro TCSPC check!
"Recommend" feature automatically fills in initial estimates for fit parameters
DeltaPro TCSPC check!
Simplified file handling, .DAS file format stores data and analyzes in one file
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Analyze up to 10,000 decays

Computer Requirements

A computer or laptop with WindowsXP or Windows 7 (32 or 64 bit), English language, with one available USB 2.0 port is all that is required to run the DeltaPro™ and DAS6 software.

For more information on the DeltaPro™ visit the DeltaPro™ Specifications page.

 
OBB has a policy of continuous product development and reserves the right to amend part numbers, descriptions and specifications without prior notice.
 
 
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