EasyLife TCSPC

DeltaPro™ Hardware

 
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DeltaPro TCSPC filter fluorometer

Shown beside the laptop is the DeltaPro sample compartment (middle), DeltaHub TCSPC interface (below right), NanoLED control box (above right), and a NanoLED (left) connected to the sample compartment. Not visible is the PPD detector connected to the back of the sample compartment.

The DeltaPro™ is a compact filter-based TCSPC fluorescence lifetime system that comes complete with PPD detection technology and software. There are number of light source options to choose from, as well as a number of accessories, such as sample handling and polarizers. At a minimum, all you need is one pulsed light source and a computer, or laptop, with a single USB port available. The system is so easy to use that it does not require installation.

Included with the System


The DeltaPro™ is a very simple system requiring no installation. It is shipped with the following components which are easily assembled by the user.

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Sample chamber with lid, containing a sample holder (with Integral stirrer and temperature sensor) and with two optical ports for the attachment of the light source and detector.
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DeltaHub™ TCSPC electronics module
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Light source(s) (NanoLED / DeltaDiode™ / SpectraLED) with appropriate controller (as specified with order)
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PPD™ picosecond photon detector
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Optional power supply (DPS-1) for longer wavelength detector versions (PPD-850, PPD-900) if ordered
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DeltaHub™ User Guide containing CD-ROM with software, drivers and instructions
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Relevant manuals
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Accessories specified at time of order

 

Three Types of pulsed Light Sources to Choose From

There is a comprehensive range of pulsed illuminators to choose from for use with the DeltaPro™. For fluorescence lifetimes you can choose from either the NanoLED™ or the DeltaDiode™ series of sources. Each series includes a mix of pulsed LED’s and lasers, with the fundamental difference the maximum repetition rate that they can operate at (1 MHz for the NanoLED™ and up to 100 MHz for the DeltaDiode). Generally speaking, the higher the repetition rate the shorter the measurement time. Each series has its own dedicated driver interface. So for example a DeltaDiode driver cannot be used with a NanoLED illuminator and vice versa.

For longer lived phosphorescence lifetimes the DeltaPro™ uses the SpectraLED series of software adjustable LED illuminators.

 

Type

Primary Benefit

Repetition Rate

Lifetime Range

Available Wavelengths (nm)

NanoLED™ LED or Laser Lowest Cost 10 KHz to 1 MHz 30 ps to 1 µs 250 to 1,310* nm
DeltaDiode™ LED or Laser Fastest 10 KHz to 100 MHz 25 ps to 1 µs 250 to 1,310* nm
SpectraLED™ LED Phosphorescence 0.1 to 3 KHz 1 µs to 1 s 265 to 1,275* nm

* Note that although these light sources are available out to long NIR wavelengths, the DeltaPro™ is only able to detect to 920 nm with the optional red extended PPD detector. If you need to measure NIR fluorescence lifetimes please look into our high end modular fluorescence and phosphorescence lifetime systems available from the HORIBA Scientific Fluorescence Group which now also includes the complete line of PTI Fluorescence Systems.

NanoLED™ Light Sources

NanoLED

The NanoLED is a novel and economical series of light sources that utilize laser diode and LED technology to generate short optical pulses over a wide range of repetition rates and wavelengths.  Optical pulses as short as 70ps can be generated at repetition rates up to 1MHz. All NanoLED sources are controlled with a NanoLED driver interface.

Each NanoLED is designed to be used at a specific wavelength and has a pulse width of 200 ps to 1.6 ns (LED dependent). For the DeltaPro NanoLED sources can be selected from 250 to 830 nm. 

Current list of available NanoLED sources for the DeltaPro™

Model

Peak wavelength (nominal)

Source type

Pulse width typical

NanoLED-250 250 ±10nm LED <1.2ns
NanoLED-260 260 ±10nm LED <1.2ns
NanoLED-265 265 ±10nm LED <1.2ns
NanoLED-270 270 ±10nm LED <1.2ns
NanoLED-280 280 ±10nm LED <1.2ns
NanoLED-290 290 ±10nm LED <1.2ns
NanoLED-295 295 ±10nm LED <1.2ns
NanoLED-300 300 ±10nm LED <1.2ns
NanoLED-310 310 ±10nm LED <1.2ns
NanoLED-320 320 ±10nm LED <1.2ns
NanoLED-330 330 ±10nm LED <1.2ns
NanoLED-340 340 ±10nm LED <1.2ns
NanoLED-350 350 ±10nm LED <1.2ns
NanoLED-360 360 ±10nm LED <1.2ns
NanoLED-370 370 ±10nm LED <1.2ns
NanoLED-375L 375 ±10nm Diode Laser <200ps
NanoLED-390 390 ±10nm LED <1.3ns
NanoLED-395L 395 ±10nm Diode Laser <200ps

Model

Peak wavelength (nominal)

Source type

Pulse width typical

NanoLED-405L 405 ±10nm Diode Laser <200ps
NanoLED-415L 415 ±10nm Diode Laser <200ps
NanoLED-425L 425 ±10nm Diode Laser <200ps
NanoLED-440L 440 ±10nm Diode Laser <200ps
NanoLED-455 455 ±10nm LED <1.3ns
NanoLED-470L 470 ±10nm Diode Laser <200ps
NanoLED-485L 485 ±10nm Diode Laser <200ps
NanoLED-510L 510 ±10nm Diode Laser <200ps
NanoLED-570 570 ±10nm LED <1.5ns
NanoLED-590 590 ±10nm LED <1.5ns
NanoLED-605 605 ±10nm LED <1.5ns
NanoLED-625 625 ±10nm LED <1.4ns
NanoLED-635L 635 ±10nm Diode Laser <200ps
NanoLED-650L 650 ±10nm Diode Laser <200ps
NanoLED-670L 670 ±10nm Diode Laser <200ps
NanoLED-740 740 ± 20nm LED <1.6ns
NanoLED-785L 85 ± 20nm Diode Laser <200ps
NanoLED-830L 830 ± 20nm Diode Laser <200ps

 

New NanoLEDs heads are continually under development — contact HORIBA Scientific for other wavelengths.

DeltaDiode™ Light Sources

DeltaDiode

The world's first 100MHz picosecond diode source with true plug-and-play interchangeable heads and USB interface!

DeltaDiode sources utilize laser diode and LED technology to generate short optical pulses over a very wide range of repetition rates and wavelengths. Optical pulses as short as 40 ps can be generated at repetition rates up to 100MHz. State of the art features such as hot-swap heads, USB control and full software integration mean the DeltaDiode delivers the highest level of performance in the most user-friendly package available today.

All DeltaDiode sources are controlled with a DeltaDiode driver interface.
DeltaDiode heads are switched using a single connection, with optimized settings stored in each head to enable the DeltaDiode software to "know" which head is connected.

Each DeltaDiode is designed to be used at a specific wavelength and has a pulse width of 40 to 1.5 ns (diode dependent). DeltaDiode sources can be selected from 250 to 830 nm for use with the DeltaPro. 

Current list of available DeltaDiode sources for the DeltaPro™

Model

Peak wavelength (nominal)

Soure type

Pulse width (Typical)

Pulse width (Max)

Peak power

Average power

Max repetition rate

DeltaDiode-250 250nm ± 10nm LED with active temp control 750ps 1.0ns 240mW 4µW 20MHz
DeltaDiode-260 260nm ± 10nm LED with active temp control 750ps 1.0ns 290mW 5µW 20MHz
DeltaDiode-270 270nm ± 10nm LED with active temp control 800ps 1.2ns 520mW 10µW 20MHz
DeltaDiode-280 280nm ± 10nm LED with active temp control 950ps 1.5ns 170mW 10µW 20MHz
DeltaDiode-290 290nm ± 10nm LED with active temp control 800ps 1.2ns 520mW 10µW 20MHz
DeltaDiode-300 300nm ± 10nm LED with active temp control 800ps 1.2ns 260mW 5µW 20MHz
DeltaDiode-310 830nm ± 10nm LED with active temp control 800ps 1.2ns 260mW 5µW 20MHz
DeltaDiode-320 320nm ± 10nm LED with active temp control 800ps 1.2ns 260mW 5µW 20MHz
DeltaDiode-330 330nm ± 10nm LED with active temp control 800ps 1.5ns 100mW 2µW 20MHz
DeltaDiode-340 340nm ± 10nm LED with active temp control 750ps 1.0ns 120mW 2µW 20MHz
DeltaDiode-350 350nm ± 10nm LED with active temp control 800ps 1.0ns 120mW 2µW 20MHz
DeltaDiode-360 360nm ± 10nm LED with active temp control 800ps 1.0ns 120mW 2µW 20MHz
DeltaDiode-370 372nm ± 10nm LED 800ps 1.2ns 100mW 2µW 20MHz
DeltaDiode-375L 375 ±7nm Laser Diode with active temp control 45ps 70ps 300mW 1.8mW 100MHz
DeltaDiode-395L 395 ±8nm Laser Diode with active temp control 70ps 90ps 400mW 3.0mW 100MHz
DeltaDiode-405L 405 ±8nm Laser Diode with active temp control 45ps 70ps 300mW 1.4mW 100MHz
DeltaDiode-415L 415 ±8nm Laser Diode with active temp control 70ps 90ps 250mW 1.7mW 100MHz
DeltaDiode-425L 425 ±8nm Laser Diode with active temp control 70ps 70ps 230mW 1.6mW 100MHz
DeltaDiode-440L 440 ±9nm Laser Diode with active temp control 60ps 70ps 400mW 3.0mW 100MHz
DeltaDiode-450L 450 ±10nm Laser Diode with active temp control 80ps 120ps 250mW 3.0mW 100MHz
DeltaDiode-470L 472 ±7nm Laser Diode with active temp control 65ps 90ps 160mW 1.5mW 100MHz
DeltaDiode-485L 485 ±10nm Laser Diode with active temp control 80ps 95ps 300mW 3.0mW 100MHz
DeltaDiode-510L 508 ±10nm Laser Diode with active temp control 110ps 140ps 100mW 1.4mW 100MHz
COMING SOON laser modules at 532 nm, 560 nm and 590 nm
DeltaDiode-635L 635 ±10nm Laser Diode with active temp control 60ps 80ps 300mW 2.0mW 100MHz
DeltaDiode-650L 650 ±15nm Laser Diode with active temp control 70ps 90ps 60mW 0.4mW 80MHz
DeltaDiode-670L 670 ±10nm Laser Diode with active temp control 75ps 90ps 30mW 0.2mW 100MHz
DeltaDiode-730L 730 ±10nm Laser Diode with active temp control 60ps 70ps 500mW 3.0mW 100MHz
DeltaDiode-785L 785 ± 10nm Laser Diode with active temp control 60ps 80ps 250mW 2mW 100MHz
DeltaDiode-830L 830nm ± 10nm Laser Diode with active temp control 50ps 70ps 200mW 0.6mW 100MHz

 

New laser heads are continually under development — contact HORIBA Scientific for other wavelengths

SpectraLED Light Sources

SpectraLED

The SpectraLED is a novel light source designed specifically for the measurement of phosphorescence lifetimes. These sources are based on LED technology and the illumination wavelengths range from the UV to the NIR.

The addition of a SpectraLED source to the DeltaPro TCSPC system enables phosphorimeter capability and the measurement of luminescence lifetimes ranging from picoseconds to seconds in one compact system.

The SpectraLED is a modern approach to measuring longer lived luminescence decays. Traditionally, phosphorescence lifetime measurements are excited using a xenon flash lamp. Xenon lamps are broadband sources and offer complete wavelength coverage from the deep UV to the NIR region. For applications where continuous wavelength tunability is not required, SpectraLED sources are a convenient alternative with the following advantages:

SpectraLED Benefits

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Operation at higher repetition rates not limited by capacitor charging times
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Software control of pulse duration and repetition rate to optimally excite the sample under investigation
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No afterglow, permitting easier interpretation of lifetimes shorter than 100 µs
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Silent operation

 

Each SpectraLED is designed to be used for a specific wavelength range and has a variable pulse width from 100 ns to milliseconds, which is controlled by the DeltaPro acquisition software. SpectraLED’s can be selected from 265 to 835 nm for use with the DeltaPro and operate at repetition rates from 0.1 to 3 KHz depending on the measurement range.

SpectraLED sources do not require a special driver. They are software driven directly from the DeltaHub TCSPC interface.

Current list of available SpectraLED sources for the DeltaPro™

Source

Peak wavelength (nm)

Typical Spectral FWHM (nm)

S-265 265 +/- 10nm 10
S-280 280 +/- 10nm 10
S-290 290 +/- 10nm 10
S-295 295 +/- 10nm 10
S-310 310 +/- 10nm 10
S-330 330 +/- 10nm 10
S-340 340 +/- 10nm 10
S-350 350 +/- 10nm 14
S-370 370 +/- 10nm 15
S-390 390 +/- 10nm 20

Source

Peak wavelength (nm)

Typical Spectral FWHM (nm)

S-415 415 +/- 10nm 20
S-460 460 +/- 10nm 30
S-495 495 +/- 10nm 30
S-535 535 +/- 20nm 30
S-560 560 +/- 10nm 15
S-590 590 +/- 10nm 15
S-605 605 +/- 15nm 20
S-625 625 +/- 10nm 25
S-740 740 +/- 10nm 25
S-830 830 +/- 10nm 50

 

New laser heads are continually under development — contact HORIBA Scientific for other wavelengths

Customer Supplied Light Sources

In addition to selecting from the current list of DeltaDiode, NanoLED and SpectraLED light sources, it is possible to make use of other customer supplied pulsed light sources with the DeltaPro™. Consult with HORIBA for use of your pulsed illuminator with the DeltaPro™ TCSPC system.  

 

Sample Compartment

The DeltaPro™ sample compartment includes a water jacketed and micro-stirred cuvette holder and our F-Link hardware communication interface which includes an interlocked sample compartment lid to protect the detector and optionally switch off the light source. There is also an optional solid sample (SC-10) holder, and an optional front face cuvette holder (SC-09).

cuvettes

Shown from left to right: Standard cuvette holder, optional solid sample holder (SC-10), and the optional front face cuvette holder (SC-09)

DeltaHub™ TCSPC Electronics Module

The DeltaPro™ is a time-domain fluorescence lifetime system that utilizes the Time-Correlated Single Photon Counting (TCSPC) technique. It collects a decay curve, allowing the user to fit the curve with comprehensive analysis software that provides many statistical tools to assess goodness of fit. TCSPC was originally developed in the early 1970's and is the best and most widely used technique for measuring fluorescence lifetimes. Continued innovation by HORIBA Scientific engineers has resulted in a fully digital TCSPC implementation ensuring that this technique remains the most powerful and versatile fluorescence lifetime measurement method even after forty years. TCSPC is clearly the best and most widely used technique for measuring lifetimes.

The DeltaPro™ incorporates HORIBA’s DeltaHub™ TCSPC electronics interface. The DeltaHub connects to the computer with a single USB connector and in addition to providing TCSPC electronics it acts as the primary interface to all available light sources, detectors, and accessories. The DeltaHub represents the latest in TCSPC development. It even allows the software to “know” what is connected to the system and assure calibration with optional F-link accessories.

Unique Benefits of DeltaHub TCSPC Electronics

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10 ns dead time timing electronics allowing for fast and efficient acquisition using high repetition rates
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Crystal locked time base ensures you never have to re-calibrate your timing electronics
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Wide lifetime measurement range from 25 ps to > 1 s

Picosecond Photon Detector (PPD™)

The DeltaPro includes our unique Picosecond Photon Detector (PPD). The PPD is a miniaturized single-photon detection module containing all the electronics necessary to detect single photons with picosecond accuracy. The DeltaPro comes standard with a PPD that can detect from 250 to 650 nm (PPD-650), and there are two other optional red extended PPD’s that can detect from 230 to 850 (PPD-850), and 230 to 920 nm (PPD-900) respectively.  The optional red extended PPD-850 and PPD-900 detectors feature integrated thermoelectric cooling of the PMT to reduce the dark count rate.

Each PPD contains a fast rise-time photomultiplier (PMT), a GHz pre-amplifier, a constant fraction discriminator (CFD) and a regulated high voltage power supply, all in one compact, fully integrated and factory optimized package. The PPD's board is housed in a precision milled, nickel-plated casing to provide the highest level of electromagnetic shielding. 
Optical coupling to the DeltaPro sample compartment is via a convenient circular bayonet mount which also provides a light-tight seal.

 

PPD-650

PPD-850

PPD-900

Spectral response 230-700 nm 230-850 nm 230-920 nm
Peak wavelength 350 nm 380 nm 490 nm
Typical dark counts <80 cps <200 cps <3000 cps
Transit Time Spread <180 ps typical at 405 nm
Thermoelectric cooling No Yes Yes
DPS-1 included Optional Yes Yes
Dimensions 77 x 107 x 48 mm
Weight 0.6 kg
Power requirements +15V @ 330mA DPS-1 DPS-1

Specifications and appearance are subject to change without notice

PPD spectral response

PPD spectral response

 
OBB has a policy of continuous product development and reserves the right to amend part numbers, descriptions and specifications without prior notice.
 
 
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08820-3097

Tel: 732-494-8660
Fax: 732-623-8129
E-mail:info.sci@horiba.com